Coding

Part:BBa_K4482002:Design

Designed by: Mustafo Mustafokulov   Group: iGEM22_NYC_Empire_State   (2022-08-25)


Human NTRK3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 111
    Illegal PstI site found at 489
    Illegal PstI site found at 742
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 111
    Illegal PstI site found at 489
    Illegal PstI site found at 742
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 111
    Illegal PstI site found at 489
    Illegal PstI site found at 742
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 111
    Illegal PstI site found at 489
    Illegal PstI site found at 742
    Illegal NgoMIV site found at 134
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 435
    Illegal SapI site found at 1089


Design Notes

The coding sequence for human NTRK3 was extracted from the original plasmid in AddGene by PCR and inserted into a new plasmid backbone to create a fusion protein with eGFP.


Source

The source of this part is an AddGene plasmid with code 20551.

References